Stool Biomarker Analysis Service
Gut Inflammation Panel
ELISA-based stool protein biomarkers for gut inflammation research
Flexible stool collection methods with optional metabolomics integration.
Available for academic, clinical, and industry research projects.
ISO 9001 & CLIA-certified laboratory • Research use only
The Gut Inflammation Biomarker Panel
Panel composition subject to finalization. Representative markers shown.
- Calprotectin
- Lactoferrin
- Lysozyme
- Secretory IgA
- Pancreatic Elastase
- Functional Beta-Glucuronidase
- Occult Blood / Hemoglobin
All markers are measured from stool samples. When ordered together with metabolomics, results can also be paired with fecal SCFA profiles (butyrate, propionate, acetate), depending on the collection method and study design.
| Biomarker | What it measures | Research context |
|---|---|---|
| Calprotectin | Neutrophil-derived protein released during intestinal inflammation | The most extensively validated fecal marker of mucosal inflammation; elevated levels are associated with active IBD and Crohn's disease in numerous clinical studies |
| Lactoferrin | Antimicrobial protein released by activated neutrophils | Well-studied fecal inflammation marker; correlates with mucosal disease activity and is used in IBD research as a complement to calprotectin |
| Lysozyme | Antimicrobial enzyme secreted by neutrophils, macrophages, and Paneth cells | A marker of innate immune activation in the gut; elevated fecal levels reflect granulocyte and macrophage infiltration and have been studied alongside calprotectin and lactoferrin in the context of intestinal inflammation and IBD activity |
| Secretory IgA | Primary antibody mediating mucosal immune defense | Reflects the activity of the gut-associated immune system; studied in the context of mucosal immunity, gut dysbiosis, and immune suppression |
| Pancreatic Elastase | Protease secreted by the exocrine pancreas and measured in stool as a proxy for pancreatic output | A well-validated, non-invasive marker of exocrine pancreatic function; low fecal levels are associated with pancreatic exocrine insufficiency (PEI) and are used in research on maldigestion, chronic pancreatitis, and related conditions |
| Beta-Glucuronidase (Functional) | Bacterial enzyme activity reflecting microbial metabolism of glucuronide conjugates in the gut | Produced primarily by gut microbiota; elevated activity has been studied in the context of dysbiosis, estrogen recycling, colon cancer risk, and toxin reactivation from conjugated compounds |
| Occult Blood / Hemoglobin | Trace amounts of blood in stool not visible to the naked eye | A clinically established screening marker for gastrointestinal bleeding; detected via fecal immunochemical testing (FIT) and used extensively in colorectal cancer screening research and the evaluation of mucosal injury |
Why Measure Gut Inflammation Proteins?
The intestinal lining and mucosal immune system release measurable proteins in response to inflammation, barrier disruption, and immune activation. These proteins can be detected in stool and are well studied in the context of inflammatory bowel disease (IBD), intestinal permeability, and mucosal immune function.
A targeted panel may provide research-grade information related to:
- Mucosal inflammation: markers such as calprotectin and lactoferrin are associated with active intestinal inflammation in published studies
- Gut barrier function: proteins such as zonulin are studied as indicators of tight junction integrity (interpretation remains an active research area)
- Mucosal immune status: secretory IgA reflects local antibody production in the gut lining
- Enterocyte integrity: I-FABP is elevated in conditions involving intestinal epithelial injury
- Systemic immune signaling: LBP is associated with bacterial translocation and downstream inflammatory responses
When combined with short-chain fatty acid (SCFA) profiles, particularly butyrate, which plays an established role in gut barrier maintenance and mucosal immunity, these markers can provide a more integrated research perspective than either measurement alone.
No individual marker should be interpreted in isolation, and results should not be used for clinical decision-making without evaluation by a qualified clinician.
Collection Methods
This panel can be performed using different stool collection approaches depending on study design, logistics, and whether paired metabolomics is required.
Raw Stool
Suitable for standard stool-based protein biomarker analysis.
Compatible with Metabolomics
IDK SAS Tube (EasySampler)
Standard stool collection into a stabilizing extraction buffer for protein immunoassays. The reference method for this panel. The EasySampler is included in the kit.
Compatible with Metabolomics
Other
Additional validated collection approaches may be used depending on study design. Please contact our team to discuss sample handling options.
Understanding Your Results
Results are reported as quantitative concentrations with reference ranges drawn from peer-reviewed literature. Out-of-range values are flagged, and a plain-language summary explains each measurement in the context of current research.
This panel may be of research interest in the context of:
- Colorectal cancer (CRC) risk factor research
- Inflammatory bowel disease (IBD) and Crohn’s disease monitoring in research settings
- Gut barrier and permeability studies
- Dietary intervention and microbiome research
This panel is a research-grade measurement service. Results are not intended to diagnose, treat, or screen for any medical condition. Any result that raises concern, particularly a pattern of elevated inflammation markers combined with disrupted SCFA levels, should be discussed with a gastroenterologist or other qualified clinician who can evaluate it in the context of the full clinical picture.
Who is this for?
For researchers and clinicians (B2B):
Purpose-built for academic and clinical research teams conducting gut health studies, dietary interventions, or longitudinal microbiome cohort studies. ELISA-based protein quantification from stool samples can support research workflows while reducing participant burden and simplifying biospecimen logistics. Arome Science’s ISO 9001 and CLIA certifications support use in regulated research environments. Bulk pricing and custom panel configurations are available upon request.
How It Works
The Gut Inflammation Panel supports stool-based protein measurement using validated collection workflows appropriate for the study design.
Collect
Collect stool using the selected collection method.
Stabilize
Process and stabilize the sample according to the chosen workflow and analytical requirements.
Measure
Measure inflammation-related proteins by ELISA.
Why this works
Samples are processed using validated handling and preparation workflows designed to preserve protein integrity for ELISA-based quantification.
When ordered together with metabolomics, results may also be paired with fecal SCFA profiles (butyrate, propionate, acetate), depending on the collection method and project setup.
Quality & Compliance
ISO 9001-certified quality management system
CLIA-certified laboratory
CLIA-certified laboratory
What is ELISA?
ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used analytical method for measuring the amount of specific proteins in biological samples. Antibodies bind selectively to a target protein, and the signal generated is proportional to its concentration. This allows for quantitative, reproducible measurement rather than a qualitative positive/negative result.
ELISA is the method behind clinically established fecal tests such as calprotectin measurement, which is a standard tool in gastroenterology research and clinical practice. The markers in this panel use the same analytical principle, applied to a broader set of gut health proteins.
All measurements are reported as concentrations against validated reference ranges. Results are intended to inform research interpretation and support conversations with qualified clinicians and do not constitute a clinical diagnosis.
Order the Gut Inflammation Panel
Available as a standalone service or as part of a broader gut health research workflow. Collection method and study design can be discussed based on project needs.
All measurements are performed in Arome Science’s ISO 9001-certified and CLIA-certified laboratory. This service is intended for research use only and is not approved for clinical diagnostic use. Results should not be used as the basis for clinical decisions without evaluation by a qualified healthcare professional.
